Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes

Dongru Qiu, Kyoko Fujita, Yuko Sakuma, Teruo Tanaka, Yoshiaki Ohashi, Hideyuki Ohshima, Masaru Tomita, Mitsuhiro Itaya

研究成果: Article査読

26 被引用数 (Scopus)


The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons (rrn) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPβ, skin element, and PBSX, as well as the insertion element IS4Bsu1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.

ジャーナルApplied and Environmental Microbiology
出版ステータスPublished - 2004 10

ASJC Scopus subject areas

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー
  • 生態学


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