Comparative studies of suppression of malignant cancer cell phenotype by antisense oligo DNA and small interfering RNA

N. Hiroi, A. Funahashi, H. Kitano

研究成果: Article査読

16 被引用数 (Scopus)

抄録

One of the distinguishing features of malignant tumor cells is the ability to proliferate in an anchorage-independent manner; methods that effectively suppress this phenotype may be applicable to the therapeutic inhibition of the malignancy of cancers. Interfering RNA is a potentially powerful tool for cancer therapy because of its specificity of target selection and remarkably high efficiency in target mRNA suppression. We studied the use of two knockdown strategies, antisense oligo DNA (AS-ODN) and small interfering RNA (siRNA), and showed how the anchorage-independent proliferation of malignant cells could be blocked efficiently. Anchorage-independent proliferation of rat fibroblasts transformed with v-src was suppressed with only a single 1-μM dose of AS-ODN; similar suppression using siRNA required treatment with 1 nM siRNA every 12 h. With our experimental system, the molecular stability of AS-ODN allowed the use of a simple treatment regimen to control the amount of the target molecule, providing that the treatment dose was sufficiently high. In comparison, siRNA treatment was effective at lower doses, but more frequent treatment was necessary to achieve the same suppression of proliferation.

本文言語English
ページ(範囲)7-12
ページ数6
ジャーナルCancer Gene Therapy
13
1
DOI
出版ステータスPublished - 2006 1

ASJC Scopus subject areas

  • 分子医療
  • 分子生物学
  • 癌研究

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