TY - JOUR
T1 - Comparative study of autoantigen profile between Colombian and Brazilian types of endemic pemphigus foliaceus by various biochemical and molecular biological techniques
AU - Hisamatsu, Yoshiko
AU - Abreu Velez, Ana Maria
AU - Amagai, Masayuki
AU - Ogawa, Marilia M.
AU - Kanzaki, Tamotsu
AU - Hashimoto, Takashi
N1 - Funding Information:
We gratefully thank Dr. Marilia M. Ogawa for generously providing us with the sera of Brazilian EPF. We thank Miss Michiyo Noge, Miss Yuko Kawano, and Miss Ayumi Suzuki for their technical assistance, and Miss Akiko Tanaka for secretarial assistance. This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and a grant from the Ministry of Health and Welfare of Japan. Dr. Hisamatsu was an awardee of the Professor Klingman Award for travel expense for the Annual Meeting for the Society of Investigative Dermatology in 2001 (Washington, DC).
PY - 2003/6
Y1 - 2003/6
N2 - Background: Besides Brazilian endemic pemphigus foliaceus (EPF), we have described another focus of EPF in Colombia. Our previous study suggested that Colombian EPF seemed to react various plakin family proteins, such as envoplakin, periplakin and BP230. Objective: To further characterize the Colombian EPF and study the difference from Brazilian EPF, we examined the antigen profile of the two types of EPF. Methods and results: Immunoblotting using normal human epidermal extracts revealed that 38% Colombian EPF sera and 25% Brazilian EPF sera showed IgG antibodies reactive with desmoglein (Dsg) 1, pemphigus foliaceus antigen. The sera of both types of EPF showed protein bands co-migrating with plakin family proteins, particularly periplakin. Immunoblotting analyses using recombinant proteins of various domains of envoplakin, periplakin and BP230 revealed that a considerable number of Colombian EPF sera reacted with recombinant proteins of periplakin, while only few Brazilian sera reacted with some of the recombinant proteins of any plakins. Enzyme-linked immunosorbent assay (ELISA) for Dsg1 and Dsg3 showed that Dsg1 was reacted by almost all sera of both types of EPF. However, unexpectedly, while none of Colombian EPF sera reacted with Dsg3, about half of Brazilian EPF sera reacted with Dsg3. Conclusion: These results suggested that the Colombian EPF is basically similar to Brazilian EPF in terms that major antigen is Dsg1, but there were some different antigen profiles between the two types of EPF.
AB - Background: Besides Brazilian endemic pemphigus foliaceus (EPF), we have described another focus of EPF in Colombia. Our previous study suggested that Colombian EPF seemed to react various plakin family proteins, such as envoplakin, periplakin and BP230. Objective: To further characterize the Colombian EPF and study the difference from Brazilian EPF, we examined the antigen profile of the two types of EPF. Methods and results: Immunoblotting using normal human epidermal extracts revealed that 38% Colombian EPF sera and 25% Brazilian EPF sera showed IgG antibodies reactive with desmoglein (Dsg) 1, pemphigus foliaceus antigen. The sera of both types of EPF showed protein bands co-migrating with plakin family proteins, particularly periplakin. Immunoblotting analyses using recombinant proteins of various domains of envoplakin, periplakin and BP230 revealed that a considerable number of Colombian EPF sera reacted with recombinant proteins of periplakin, while only few Brazilian sera reacted with some of the recombinant proteins of any plakins. Enzyme-linked immunosorbent assay (ELISA) for Dsg1 and Dsg3 showed that Dsg1 was reacted by almost all sera of both types of EPF. However, unexpectedly, while none of Colombian EPF sera reacted with Dsg3, about half of Brazilian EPF sera reacted with Dsg3. Conclusion: These results suggested that the Colombian EPF is basically similar to Brazilian EPF in terms that major antigen is Dsg1, but there were some different antigen profiles between the two types of EPF.
KW - Autoimmune bullous disease
KW - Desmocollin
KW - Desmoglein
KW - Desmosome
KW - Endemic pemphigus foliaceus
KW - Enzyme-linked immunosorbent assay
KW - Immunoblotting
KW - Plakin family protein
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U2 - 10.1016/S0923-1811(03)00034-3
DO - 10.1016/S0923-1811(03)00034-3
M3 - Article
C2 - 12788527
AN - SCOPUS:0038547753
VL - 32
SP - 33
EP - 41
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 1
ER -