Comparison of various bone marrow fractions in the ability to participate in vascular remodeling after mechanical injury

Makoto Sahara, Masataka Sata, Yumi Matsuzaki, Kimie Tanaka, Toshihiro Morita, Yasunobu Hirata, Hideyuki Okano, Ryozo Nagai

研究成果: Article査読

44 被引用数 (Scopus)

抄録

In contrast to conventional assumption, recent reports propose the possibility that hematopoietic stem cells (HSCs) may have broader potential to differentiate into various cell types. Here, we tested the pluripotency of HSCs by comparing vascular lesions induced by mechanical injury after bone marrow reconstitution with total bone marrow (TBM) cells, C-KH+ Sca-1 + Lin- (KSL) cells, or a single HSC cell (Tip-SP CD34-KSL cell, CD34- c-Kit+ Sca-1+ Lin- cell with the strongest dyeefflux activity) harboring green fluorescent protein (GFP). The lesions contained a significant number of GFP-positive cells in the TBM and KSL groups, whereas GFP-positive cells were rarely detected in the HSC group. These results suggest that transdifferentiation of a highly purified HSC seems to be a rare event, if it occurs at all, whereas bone marrow cells including the KSL fraction can give rise to vascular cells that substantially contribute to repair or lesion formation after mechanical injury.

本文言語English
ページ(範囲)874-878
ページ数5
ジャーナルStem Cells
23
7
DOI
出版ステータスPublished - 2005 8月

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

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