Complex formation of the neuron-specific ELAV-like Hu RNA-binding proteins

Katsumi Kasashima, Eiji Sakashita, Kuniaki Saito, Hiroshi Sakamoto

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Hu proteins are RNA-binding proteins that are the vertebrate homologs of Drosophila ELAV, and are implicated in stabilization or enhanced translation of specific mRNAs with AU-rich elements (AREs) in the 3′-untranslated region. Here, using the yeast two-hybrid system, we show that neuron-specific Hu proteins can interact with themselves. Immuno-precipitation assays demonstrated that the interaction between Hu proteins occurs in mammalian cells and is strongly enhanced in the presence of cellular RNA. Furthermore, using in situ chemical crosslinking assays, we found that HuD, one of the neuron-specific Hu proteins, multimerizes in cells. The crosslinked HuD multimers retain specific RNA-binding ability and can interact with additional Hu proteins. Consistent with this biochemical property, HuD showed granular distribution in two neurogenic cell lines. These results suggest that the RNA-bound form of HuD multimerizes cooperatively to form a specific granular structure that may serve as a site of post-transcriptional regulation of ARE-containing mRNAs.

本文言語English
ページ(範囲)4519-4526
ページ数8
ジャーナルNucleic acids research
30
20
DOI
出版ステータスPublished - 2002 10 15
外部発表はい

ASJC Scopus subject areas

  • 遺伝学

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