Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia

Keiko Hamano-Hasegawa, Miyuki Morozumi, Eiichi Nakayama, Naoko Chiba, Somay Y. Murayama, Reiko Takayanagi, Satoshi Iwata, Keisuke Sunakawa, Kimiko Ubukata, Shinobu Ishizawa, Mika Numata, Michiyo Kojima, Masato Nonoyama, Haruo Kuroki, Koichi Shimizu, Masaaki Kobayashi, Kota Saito, Masahiko Nitta, Naohisa Kawamura, Ritsuko SakaiShigeru Ohnari, Takao Morinobu, Takeshi Tajima, Tomohiro Oishi

研究成果: Article査読

94 被引用数 (Scopus)

抄録

We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13 respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and B; parainfluenza virus (PIV) 1, 2, and 3; adenovirus; rhinovirus (RV); enterovirus; coronavirus (OC43); human metapneumovirus (hMPV); and human bocavirus (HBoV). The new method for detection of these viruses was applied simultaneously with real-time PCR for the detection of six bacterial pathogens in clinical samples from 1700 pediatric patients with community-acquired pneumonia (CAP). Of all the patients, 32.5% were suspected to have single bacterial infections; 1.9%, multiple bacterial infections; 15.2%, coinfections of bacteria and viruses; 25.8%, single viral infections; and 2.1%, multiple viral infections. In the remaining 22.6%, the etiology was unknown. The breakdown of suspected causative pathogens was as follows: 24.4% were Streptococcus pneumoniae, 14.8% were Mycoplasma pneumoniae, 11.3% were Haemophilus influenzae, and 1.4% were Chlamydophila pneumoniae. The breakdown of viruses was as follows: 14.5% were RV, 9.4% were RSV, 7.4% were hMPV, 7.2% were PIV, and 2.9% were HBoV. The new method will contribute to advances in the accuracy of diagnosis and should also result in the appropriate use of antimicrobials.

本文言語English
ページ(範囲)424-432
ページ数9
ジャーナルJournal of Infection and Chemotherapy
14
6
DOI
出版ステータスPublished - 2008 12

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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