Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells

Kazutaka Terahara, Masato Yoshida, Osamu Igarashi, Tomonori Nochi, Gemilson Soares Pontes, Koji Hase, Hiroshi Ohno, Shiho Kurokawa, Mio Mejima, Naoko Takayama, Yoshikazu Yuki, Anson W. Lowe, Hiroshi Kiyono

研究成果: Article査読

136 被引用数 (Scopus)

抄録

Separate populations of M cells have been detected in the follicle-associated epithelium of. Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.

本文言語English
ページ(範囲)7840-7846
ページ数7
ジャーナルJournal of Immunology
180
12
DOI
出版ステータスPublished - 2008
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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