Computer analysis of potential stem structures of rRNA operons in various procaryote genomes

Rintaro Saito, Yousuke Ozawa, Naoki Kuzuno, Masaru Tomita

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The processing of 16S rRNA and 23S rRNA by RNase III in E.coli is known to involve stem structures formed by both ends of the rRNA. Indeed, complementary nucleotide sequences are usually found at both ends of 16S rRNA and 23S rRNA. However, whether or not this phenomenon exists in various other bacteria has not yet been adequately studied. We have conducted computer analyses of potential stem structures of rRNA operons in 12 bacterial and 3 archaeal genomes, and compared characteristics of the stem structures among these species. We systematically computed free energy values by exhaustively 'annealing' sequences around the 5′ end and sequences around the 3′ end of both 16S rRNA and 23S rRNA genes, in order to predict potential stem structures. The results suggest that rRNAs in most species form stem structures at both ends. Some species, such as A.aeolicus, seem to form unusually stable stem structures. On the other hand, some rRNAs, such as rRNAs of D.radiodurans, seem not to form solid stem structures. This suggests that rRNA processing in those species must employ a reliable targeting mechanism other than recognizing stem structures by RNase III.

本文言語English
ページ(範囲)217-222
ページ数6
ジャーナルGene
259
1-2
DOI
出版ステータスPublished - 2000 12 23

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Computer analysis of potential stem structures of rRNA operons in various procaryote genomes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル