Computer-guided design of optimal microbial consortia for immune system modulation

Richard R. Stein, Takeshi Tanoue, Rose L. Szabady, Shakti K. Bhattarai, Bernat Olle, Jason M. Norman, Wataru Suda, Kenshiro Oshima, Masahira Hattori, Georg K. Gerber, Chris Sander, Kenya Honda, Vanni Bucci

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Manipulation of the gut microbiota holds great promise for the treatment of diseases. However, a major challenge is the identification of therapeutically potent microbial consortia that colonize the host effectively while maximizing immunologic outcome. Here, we propose a novel workflow to select optimal immune-inducing consortia from microbiome compositicon and immune effectors measurements. Using published and newly generated microbial and regulatory T-cell (Treg) data from germ-free mice, we estimate the contributions of twelve Clostridia strains with known immune-modulating effect to Treg induction. Combining this with a longitudinal data-constrained ecological model, we predict the ability of every attainable and ecologically stable subconsortium in promoting Treg activation and rank them by the Treg Induction Score (TrIS). Experimental validation of selected consortia indicates a strong and statistically significant correlation between predicted TrIS and measured Treg. We argue that computational indexes, such as the TrIS, are valuable tools for the systematic selection of immune-modulating bacteriotherapeutics.

本文言語English
論文番号e30916
ジャーナルeLife
7
DOI
出版ステータスPublished - 2018 4 17

ASJC Scopus subject areas

  • 神経科学(全般)
  • 免疫学および微生物学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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