Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster

Michal Jaroš, Tomoyoshi Soga, Tom van de Goor, Bohuslav Gaš

研究成果: Article

42 引用 (Scopus)

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A simple rule stating that the signal in conductivity detection in capillary zone electrophoresis is proportional to the difference between the analyte mobility and mobility of the background electrolyte (BGE) co-ion is valid only for systems with fully ionized electrolytes. In zone electrophoresis systems with weak electrolytes both conductivity signal and electromigration dispersion of analyte peaks depend on the conductivity and pH effects. This allows optimization of the composition of BGEs to give a good conductivity signal of analytes while still keeping electromigration dispersion near zero, regardless of the injected amount of sample. The demands to achieve minimum electromigration dispersion and high sensitivity in conductivity detection can be accomplished at the same time. PeakMaster software is used for inspection of BGEs commonly used for separation of sugars (carbohydrates, saccharides) at highly alkaline pH. It is shown that the terms direct and indirect conductivity detection are misleading and should not be used.

元の言語English
ページ(範囲)1948-1953
ページ数6
ジャーナルELECTROPHORESIS
26
発行部数10
DOI
出版物ステータスPublished - 2005 5 1

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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