Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, N. Miki, S. Takeuchi

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

This paper proposes a confocal laser scanning microscopic (CLSM) observation system for liposomes and cells that are mechanically trapped on a parylene microfilter. CLSM allowed us to acquire three-dimensional and highly sensitive fluorescent images, which we exploited to evaluate the deformability of liposomes with or without cholesterol, the amount of calcein that was introduced into the liposomes via membrane proteins, and the contact areas of the adjacent cells. The proposed system is readily applicable to analyze a single liposome and cells.

本文言語English
ホスト出版物のタイトル2013 Transducers and Eurosensors XXVII
ホスト出版物のサブタイトルThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
ページ416-417
ページ数2
DOI
出版ステータスPublished - 2013
イベント2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
継続期間: 2013 6 162013 6 20

出版物シリーズ

名前2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CountrySpain
CityBarcelona
Period13/6/1613/6/20

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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