Constructing a multi-scan synchrotron X-ray microscope to study the function of osteocyte canaliculi in mouse bone

Nobuhito Nango, Shogo Kubota, Wataru Yashiro, Atsushi Momose, Yasunari Takada, Koichi Matsuo

研究成果: Conference contribution

抄録

Formulating a multi-scan method applied to an X-ray microscope CT with synchrotron radiation, we attempted to analyze the 3D functional structure of osteocyte canaliculi inside the cortical bone of a mouse tibia. We employed a two-method combination to scan the same position of the specimen. To extract the internal bone canalicular structure, we first combined a Talbot interferometer with an X-ray microscope, and applied a differential phase imaging method to measure the absolute value of bone mineral around the canaliculi. Next, we used the X-ray microscope without the Talbot interferometer under a defocus condition, moving the specimen toward the zone plate by 6 mm. This defocus contrast method visualizes the canaliculi by emphasizing the edges of the bone. We performed CT scans by the two configurations and precisely aligned resultant 3D images so that the same position in the specimen is compared. We could extract the osteocyte canaliculi and evaluate the mineral density of their surroundings. The degree of mineralization varied for each osteocyte lacuna and canaliculus. The multi-scan microscopic X-ray CT is a powerful tool for analyzing bone mineralization.

本文言語English
ホスト出版物のタイトルInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings
ページ199-204
ページ数6
DOI
出版ステータスPublished - 2012
イベントInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings, XNPIG 2012 - Tokyo, Japan
継続期間: 2012 3 52012 3 7

出版物シリーズ

名前AIP Conference Proceedings
1466
ISSN(印刷版)0094-243X
ISSN(電子版)1551-7616

Other

OtherInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings, XNPIG 2012
国/地域Japan
CityTokyo
Period12/3/512/3/7

ASJC Scopus subject areas

  • 物理学および天文学(全般)

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