Construction and Characterization of Single-Gene Chromosomes in Saccharomyces cerevisiae

Kazuo Yamagishi, Minetaka Sugiyama, Yoshinobu Kaneko, Masafumi Nishizawa, Satoshi Harashima

研究成果: Article査読

6 被引用数 (Scopus)


Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

ジャーナルJournal of Bioscience and Bioengineering
出版ステータスPublished - 2008 12 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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