Construction of a metagenomic library for the marine sponge Halichondria okadai

Takahiro Abe, Fatma Pinar Sahin, Kiyotaka Akiyama, Takayuki Naito, Mizoe Kishigami, Kenji Miyamoto, Yasufumi Sakakibara, Daisuke Uemura

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454- pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.

本文言語English
ページ(範囲)633-639
ページ数7
ジャーナルBioscience, Biotechnology and Biochemistry
76
4
DOI
出版ステータスPublished - 2012

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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