Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump

Yuji Morita, Yukiko Komori, Takehiko Mima, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article

62 引用 (Scopus)

抄録

We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

元の言語English
ページ(範囲)139-143
ページ数5
ジャーナルFEMS Microbiology Letters
202
発行部数1
DOI
出版物ステータスPublished - 2001 8 7
外部発表Yes

Fingerprint

Operon
Pseudomonas aeruginosa
Acriflavine
Ethidium
Fluoroquinolones
Chloramphenicol
Erythromycin
Tetracycline
Pharmaceutical Preparations
rhodamine 6G
tetraphenylphosphonium

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

これを引用

Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1 : MexCD-OprJ is an inducible pump. / Morita, Yuji; Komori, Yukiko; Mima, Takehiko; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

:: FEMS Microbiology Letters, 巻 202, 番号 1, 07.08.2001, p. 139-143.

研究成果: Article

@article{02f379d8f3664bcaaff2d477a7131823,
title = "Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1: MexCD-OprJ is an inducible pump",
abstract = "We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.",
keywords = "A series of deletion mutants, Drug specificity, Mex multidrug efflux pump, MexCD-OprM, Pseudomonas aeruginosa",
author = "Yuji Morita and Yukiko Komori and Takehiko Mima and Teruo Kuroda and Tohru Mizushima and Tomofusa Tsuchiya",
year = "2001",
month = "8",
day = "7",
doi = "10.1016/S0378-1097(01)00314-7",
language = "English",
volume = "202",
pages = "139--143",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Construction of a series of mutants lacking all of the four major mex operons for multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1

T2 - MexCD-OprJ is an inducible pump

AU - Morita, Yuji

AU - Komori, Yukiko

AU - Mima, Takehiko

AU - Kuroda, Teruo

AU - Mizushima, Tohru

AU - Tsuchiya, Tomofusa

PY - 2001/8/7

Y1 - 2001/8/7

N2 - We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

AB - We constructed a series of deletion mutants lacking all of the four major mex operons for Mex multidrug efflux pumps or possessing each one of the operons from Pseudomonas aeruginosa PAO1. The drug specificity of MexAB-OprM, MexXY-OprM and MexCD-OprJ was investigated. Surprisingly, we found that the MexCD-OprJ was an inducible pump, inducers of which were tetraphenylphosphonium chloride, ethidium bromide, rhodamine 6G and acriflavine. Fluoroquinolones, chloramphenicol, erythromycin and tetracycline were not inducers although they were substrates of MexCD-OprJ.

KW - A series of deletion mutants

KW - Drug specificity

KW - Mex multidrug efflux pump

KW - MexCD-OprM

KW - Pseudomonas aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=0035822580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035822580&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(01)00314-7

DO - 10.1016/S0378-1097(01)00314-7

M3 - Article

C2 - 11506922

AN - SCOPUS:0035822580

VL - 202

SP - 139

EP - 143

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -