TY - JOUR
T1 - Construction of in vitro cardiomyocyte electrical conduction line
AU - Kurotsu, Mariko
AU - Machida, Naoki
AU - Ogawa, Emiyu
AU - Arai, Tsunenori
PY - 2014/8/17
Y1 - 2014/8/17
N2 - In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO2. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.
AB - In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO2. Cardiomyocyte cultivate concentration were 2.0-8.0×105 cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca2+ probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×105 cells/ml. In 4 days cultivated 4.0×105 cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.
KW - Electrophysiology
KW - Myocardium
KW - Photodynamic therapy
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U2 - 10.11239/jsmbe.52.O-123
DO - 10.11239/jsmbe.52.O-123
M3 - Article
AN - SCOPUS:84939444084
VL - 52
SP - O-123-O-124
JO - BME = Bio medical engineering / henshu, Nihon ME Gakkai
JF - BME = Bio medical engineering / henshu, Nihon ME Gakkai
SN - 1347-443X
ER -