In order to study condition of arrhythmia treatment by a photosensitization reaction, we developed in vitro cardiomyocyte electrical conduction (EC) line to study EC block by photosensitization reaction. Cardiomyocytes were cultivated on 10 mmφ cover glasses. Cultivated and non-cultivated width were 60 and 300 μm, respectively; for 3-4 days, 37C°, 5% CO<inf>2</inf>. Cardiomyocyte cultivate concentration were 2.0-8.0×10<sup>5</sup> cells/ml. EC of cardiomyocite was measured by 5 μM Fluo-4 AM (Ca<sup>2+</sup> probe) with a confocal microscope system. Frame rate of EM-CCD was 400 ms. Cadiomyocyte was cultivated in a confluent line by 4.0×10<sup>5</sup> cells/ml. In 4 days cultivated 4.0×10<sup>5</sup> cells/ml cardiomyocite line, 1 /s synchronized pulsation was measured as Fluo-4 AM intensity change. Cross correlation coefficient was about 0.8. This result indicated that cardiomyocyte EC was occurred.
|ジャーナル||Transactions of Japanese Society for Medical and Biological Engineering|
|出版ステータス||Published - 2014 8月 17|
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