Construction of yeast secretion vectors designed for production of mature proteins using the signal sequence of yeast invertase

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Two kinds of yeast secretion vectors were constructed by site-directed mutagenesis of the invertase signal sequence and ligation of synthetic oligonucleotides coding appropriate signals. Each has a cloning site for a foreign gene preceded by a sequence encoding either the signal peptide cleavage site or a Lys-Arg sequence which is a cleavage site for the product of the KEX2 gene. Both vectors were able to direct the expression and secretion of mouse amylase. One of them has a SalI site within the signal sequence, and an attempt to clone sequences enhancing secretion of amylase with this vector is reported.

本文言語English
ページ(範囲)317-322
ページ数6
ジャーナルApplied Microbiology and Biotechnology
32
3
DOI
出版ステータスPublished - 1989 12 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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