Continuous measurement of shear-induced platelet aggregation

Mayumi Fukuyama, Kiyotaka Sakai, Ichiro Itagaki, Koichi Kawano, Mitsuru Murata, Yohko Kawai, Kiyoaki Watanabe, Makoto Handa, Yasuo Ikeda

研究成果: Article

25 引用 (Scopus)

抄録

A mechanical device for continuous recording of shear-induced platelet aggregation (SIPA) has been developed using a turbidometric technique. The device consists of three components: light source, a thermostated cone-plate streaming chamber and optical detection unit. There was a good correlation between platelet count and transmitted light intensity under the conditions in which no activation of platelets occurred. When platelets were exposed to shear stress at 37°C, transmitted light intensity increased with time. Scanning electronmicroscopic examinations revealed that more prominent platelet aggregation was demonstrated when light intensity increased. Reproducible recording of SIPA was obtained when experiments were repeated using the same sample. No change in light intensity was observed when formalin-fixed platelets were sheared. Both PGE1, and anti-GPIIb/IIIa mouse monoclonal antibody, AP2, showed complete inhibition of SIPA. It is indicated that our device for continuous measurement of SIPA is an important new tool to study one aspect of platelet functions.

元の言語English
ページ(範囲)253-260
ページ数8
ジャーナルThrombosis Research
54
発行部数3
DOI
出版物ステータスPublished - 1989 5 1

Fingerprint

Platelet Aggregation
Light
Blood Platelets
Equipment and Supplies
Alprostadil
Platelet Activation
Platelet Count
Formaldehyde
Monoclonal Antibodies

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

これを引用

Fukuyama, M., Sakai, K., Itagaki, I., Kawano, K., Murata, M., Kawai, Y., ... Ikeda, Y. (1989). Continuous measurement of shear-induced platelet aggregation. Thrombosis Research, 54(3), 253-260. https://doi.org/10.1016/0049-3848(89)90233-8

Continuous measurement of shear-induced platelet aggregation. / Fukuyama, Mayumi; Sakai, Kiyotaka; Itagaki, Ichiro; Kawano, Koichi; Murata, Mitsuru; Kawai, Yohko; Watanabe, Kiyoaki; Handa, Makoto; Ikeda, Yasuo.

:: Thrombosis Research, 巻 54, 番号 3, 01.05.1989, p. 253-260.

研究成果: Article

Fukuyama, M, Sakai, K, Itagaki, I, Kawano, K, Murata, M, Kawai, Y, Watanabe, K, Handa, M & Ikeda, Y 1989, 'Continuous measurement of shear-induced platelet aggregation', Thrombosis Research, 巻. 54, 番号 3, pp. 253-260. https://doi.org/10.1016/0049-3848(89)90233-8
Fukuyama M, Sakai K, Itagaki I, Kawano K, Murata M, Kawai Y その他. Continuous measurement of shear-induced platelet aggregation. Thrombosis Research. 1989 5 1;54(3):253-260. https://doi.org/10.1016/0049-3848(89)90233-8
Fukuyama, Mayumi ; Sakai, Kiyotaka ; Itagaki, Ichiro ; Kawano, Koichi ; Murata, Mitsuru ; Kawai, Yohko ; Watanabe, Kiyoaki ; Handa, Makoto ; Ikeda, Yasuo. / Continuous measurement of shear-induced platelet aggregation. :: Thrombosis Research. 1989 ; 巻 54, 番号 3. pp. 253-260.
@article{b9fa139c7f4847e990f017d0df36f688,
title = "Continuous measurement of shear-induced platelet aggregation",
abstract = "A mechanical device for continuous recording of shear-induced platelet aggregation (SIPA) has been developed using a turbidometric technique. The device consists of three components: light source, a thermostated cone-plate streaming chamber and optical detection unit. There was a good correlation between platelet count and transmitted light intensity under the conditions in which no activation of platelets occurred. When platelets were exposed to shear stress at 37°C, transmitted light intensity increased with time. Scanning electronmicroscopic examinations revealed that more prominent platelet aggregation was demonstrated when light intensity increased. Reproducible recording of SIPA was obtained when experiments were repeated using the same sample. No change in light intensity was observed when formalin-fixed platelets were sheared. Both PGE1, and anti-GPIIb/IIIa mouse monoclonal antibody, AP2, showed complete inhibition of SIPA. It is indicated that our device for continuous measurement of SIPA is an important new tool to study one aspect of platelet functions.",
keywords = "cone and plate viscometer, continuous measurement, optical method, shear-induced platelet aggregation",
author = "Mayumi Fukuyama and Kiyotaka Sakai and Ichiro Itagaki and Koichi Kawano and Mitsuru Murata and Yohko Kawai and Kiyoaki Watanabe and Makoto Handa and Yasuo Ikeda",
year = "1989",
month = "5",
day = "1",
doi = "10.1016/0049-3848(89)90233-8",
language = "English",
volume = "54",
pages = "253--260",
journal = "Thrombosis Research",
issn = "0049-3848",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Continuous measurement of shear-induced platelet aggregation

AU - Fukuyama, Mayumi

AU - Sakai, Kiyotaka

AU - Itagaki, Ichiro

AU - Kawano, Koichi

AU - Murata, Mitsuru

AU - Kawai, Yohko

AU - Watanabe, Kiyoaki

AU - Handa, Makoto

AU - Ikeda, Yasuo

PY - 1989/5/1

Y1 - 1989/5/1

N2 - A mechanical device for continuous recording of shear-induced platelet aggregation (SIPA) has been developed using a turbidometric technique. The device consists of three components: light source, a thermostated cone-plate streaming chamber and optical detection unit. There was a good correlation between platelet count and transmitted light intensity under the conditions in which no activation of platelets occurred. When platelets were exposed to shear stress at 37°C, transmitted light intensity increased with time. Scanning electronmicroscopic examinations revealed that more prominent platelet aggregation was demonstrated when light intensity increased. Reproducible recording of SIPA was obtained when experiments were repeated using the same sample. No change in light intensity was observed when formalin-fixed platelets were sheared. Both PGE1, and anti-GPIIb/IIIa mouse monoclonal antibody, AP2, showed complete inhibition of SIPA. It is indicated that our device for continuous measurement of SIPA is an important new tool to study one aspect of platelet functions.

AB - A mechanical device for continuous recording of shear-induced platelet aggregation (SIPA) has been developed using a turbidometric technique. The device consists of three components: light source, a thermostated cone-plate streaming chamber and optical detection unit. There was a good correlation between platelet count and transmitted light intensity under the conditions in which no activation of platelets occurred. When platelets were exposed to shear stress at 37°C, transmitted light intensity increased with time. Scanning electronmicroscopic examinations revealed that more prominent platelet aggregation was demonstrated when light intensity increased. Reproducible recording of SIPA was obtained when experiments were repeated using the same sample. No change in light intensity was observed when formalin-fixed platelets were sheared. Both PGE1, and anti-GPIIb/IIIa mouse monoclonal antibody, AP2, showed complete inhibition of SIPA. It is indicated that our device for continuous measurement of SIPA is an important new tool to study one aspect of platelet functions.

KW - cone and plate viscometer

KW - continuous measurement

KW - optical method

KW - shear-induced platelet aggregation

UR - http://www.scopus.com/inward/record.url?scp=0024315819&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024315819&partnerID=8YFLogxK

U2 - 10.1016/0049-3848(89)90233-8

DO - 10.1016/0049-3848(89)90233-8

M3 - Article

C2 - 2501904

AN - SCOPUS:0024315819

VL - 54

SP - 253

EP - 260

JO - Thrombosis Research

JF - Thrombosis Research

SN - 0049-3848

IS - 3

ER -