Continuous optical measurement system of hemolysis during a photosensitization reaction using absorption spectrum

R. Hamada, E. Ogawa, T. Arai

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

To investigate hemolysis phenomena during a photosensitization reaction with the reaction condition continuously and simultaneously for a safety assessment of hemolysis side effect, we constructed an optical system to measure blood sample absorption spectrum during the reaction. Hemolysis degree might be under estimated in general evaluation methods because there is a constant oxygen pressure assumption in spite of oxygen depression take place. By investigating hemoglobin oxidation and oxygen desorption dynamics obtained from the contribution of the visible absorption spectrum and multiple regression analysis, both the hemolysis phenomena and its oxygen environment might be obtained with time. A 664 nm wavelength laser beam for the reaction excitation and 475-650 nm light beam for measuring the absorbance spectrum were arranged perpendicularly crossing. A quartz glass cuvette with 1×10 mm in dimensions for the spectrum measurement was located at this crossing point. A red blood cells suspension medium was arranged with low hematocrit containing 30 μg/ml talaporfin sodium. This medium was irradiated up to 40 J/cm2. The met-hemoglobin, oxygenatedhemoglobin, and deoxygenated-hemoglobin concentrations were calculated by a multiple regression analysis from the measured spectra. We confirmed the met-hemoglobin concentration increased and oxygen saturation decreased with the irradiation time, which seems to indicate the hemolysis progression and oxygen consumption, respectively. By using our measuring system, the hemolysis progression seems to be obtained with oxygen environment information.

本文言語English
ホスト出版物のタイトルOptical Interactions with Tissue and Cells XXIX
編集者E. Duco Jansen, Hope Thomas Beier
出版社SPIE
ISBN(電子版)9781510614697
DOI
出版ステータスPublished - 2018
イベントOptical Interactions with Tissue and Cells XXIX 2018 - San Francisco, United States
継続期間: 2018 1 292018 1 30

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
10492
ISSN(印刷版)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXIX 2018
CountryUnited States
CitySan Francisco
Period18/1/2918/1/30

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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