Contributions of high mobility group box protein in experimental and clinical acute lung injury

Hiroshi Ueno, Tomoyuki Matsuda, Satoru Hashimoto, Fumimasa Amaya, Yoshihiro Kitamura, Masaki Tanaka, Atsuko Kobayash, Ikuro Maruyama, Shingo Yamada, Naoki Hasegawa, Junko Soejima, Hidefumi Koh, Akitoshi Ishizaka

研究成果: Article査読

317 被引用数 (Scopus)

抄録

This study was performed to examine the putative role of high mobility group box (HMGB) protein in the pathogenesis of acute lung injury (ALI). Observations were made (7) in 21 patients who were septic with ALI and 15 patients with normal lung function and (2) in a mouse model 24 hours after intratracheal instillation of lipopolysaccharide (LPS). The concentrations of HMGB1 were increased in plasma and lung epithelial lining fluid of patients with ALI and mice instilled with LPS. LPS-induced ALI was mitigated by anti-HMGB1 antibody. Although this protein was not detected in the plasma of control humans or mice, the concentrations of HMGB1 in lung epithelial lining fluid or in bronchoalveolar lavage fluid were unexpectedly high. The nuclear expression of HMGB1 was apparent in epithelial cells surrounding terminal bronchioles in normal mice, whereas its nuclear and cytoplasmic expression was observed in alveolar macrophages in LPS-instilled mice. Lung instillation of HMGB2 did not cause as much inflammation as HMGB1. Extracellular HMGB1 may play a key role in the pathogenesis of clinical and experimental ALI. However, its expression in normal airways is noteworthy and suggests that it also plays a physiologic role in the lung.

本文言語English
ページ(範囲)1310-1316
ページ数7
ジャーナルAmerican journal of respiratory and critical care medicine
170
12
DOI
出版ステータスPublished - 2004 12 15
外部発表はい

ASJC Scopus subject areas

  • 呼吸器内科
  • 集中医療医学

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