Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli

Tohru Mizushima, Rimiko Koyanagi, Emi Suzuki, Arihiro Tomura, Kazuhiro Kutsukake, Takeyoshi Miki, Kazuhisa Sekimizu

研究成果: Article査読

17 被引用数 (Scopus)

抄録

We reported elsewhere that mutation in the pgsA gene, responsible for the synthesis of phosphatidylglycerol, repressed the synthesis of flagellin and caused the loss of motility of Escherichia coli (Tomura et al., FEBS Letters 329, 287-290, 1993). We now describe evidence for a decrease in promoter activity of the flhD gene, a master gene for flagellum synthesis, in the pgsA3 mutant. We constructed a plasmid with a promoter region of the flhD gene connected with the structure region of the lacZ gene. The activity of β-galactosidase in the extract prepared from the pgsA3 mutant harboring the fusion plasmid was 30% of that in the wild type cells. This result means that phosphatidylglycerol is likely to be required for the initiation of transcription of the flhD gene. We also found that the motility-less phenotype of the mutant was partially suppressed by elevating incubation temperature. This suppression is caused by restoration of transcription of the flhD gene by high temperature. As the content of phosphatidylglycerol did not increase by elevating incubation temperature, we proposed that this suppression is caused by alternation of a physical structure of phospholipid bilayers in cytoplasmic membranes.

本文言語English
ページ(範囲)397-401
ページ数5
ジャーナルBBA - General Subjects
1245
3
DOI
出版ステータスPublished - 1995 12月 14

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学

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