Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli

Tohru Mizushima, Rimiko Koyanagi, Emi Suzuki, Arihiro Tomura, Kazuhiro Kutsukake, Takeyoshi Miki, Kazuhisa Sekimizu

研究成果: Article

17 引用 (Scopus)

抄録

We reported elsewhere that mutation in the pgsA gene, responsible for the synthesis of phosphatidylglycerol, repressed the synthesis of flagellin and caused the loss of motility of Escherichia coli (Tomura et al., FEBS Letters 329, 287-290, 1993). We now describe evidence for a decrease in promoter activity of the flhD gene, a master gene for flagellum synthesis, in the pgsA3 mutant. We constructed a plasmid with a promoter region of the flhD gene connected with the structure region of the lacZ gene. The activity of β-galactosidase in the extract prepared from the pgsA3 mutant harboring the fusion plasmid was 30% of that in the wild type cells. This result means that phosphatidylglycerol is likely to be required for the initiation of transcription of the flhD gene. We also found that the motility-less phenotype of the mutant was partially suppressed by elevating incubation temperature. This suppression is caused by restoration of transcription of the flhD gene by high temperature. As the content of phosphatidylglycerol did not increase by elevating incubation temperature, we proposed that this suppression is caused by alternation of a physical structure of phospholipid bilayers in cytoplasmic membranes.

元の言語English
ページ(範囲)397-401
ページ数5
ジャーナルBBA - General Subjects
1245
発行部数3
DOI
出版物ステータスPublished - 1995 12 14
外部発表Yes

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Phosphatidylglycerols
Escherichia coli
Genes
Gene Expression
Temperature
Transcription
Plasmids
Galactosidases
Flagellin
Lac Operon
Flagella
Genetic Promoter Regions
Phospholipids
Restoration
Cell Membrane
Fusion reactions
Phenotype
Mutation
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

Mizushima, T., Koyanagi, R., Suzuki, E., Tomura, A., Kutsukake, K., Miki, T., & Sekimizu, K. (1995). Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli. BBA - General Subjects, 1245(3), 397-401. https://doi.org/10.1016/0304-4165(95)00114-X

Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli. / Mizushima, Tohru; Koyanagi, Rimiko; Suzuki, Emi; Tomura, Arihiro; Kutsukake, Kazuhiro; Miki, Takeyoshi; Sekimizu, Kazuhisa.

:: BBA - General Subjects, 巻 1245, 番号 3, 14.12.1995, p. 397-401.

研究成果: Article

Mizushima, T, Koyanagi, R, Suzuki, E, Tomura, A, Kutsukake, K, Miki, T & Sekimizu, K 1995, 'Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli', BBA - General Subjects, 巻. 1245, 番号 3, pp. 397-401. https://doi.org/10.1016/0304-4165(95)00114-X
Mizushima T, Koyanagi R, Suzuki E, Tomura A, Kutsukake K, Miki T その他. Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli. BBA - General Subjects. 1995 12 14;1245(3):397-401. https://doi.org/10.1016/0304-4165(95)00114-X
Mizushima, Tohru ; Koyanagi, Rimiko ; Suzuki, Emi ; Tomura, Arihiro ; Kutsukake, Kazuhiro ; Miki, Takeyoshi ; Sekimizu, Kazuhisa. / Control by phosphatidylglycerol of expression of the flhD gene in Escherichia coli. :: BBA - General Subjects. 1995 ; 巻 1245, 番号 3. pp. 397-401.
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AU - Sekimizu, Kazuhisa

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