Control of burn wound sepsis in rats by methylene blue-mediated photodynamic treatment

Hiroyuki Hasegawa, Shunichi Sato, Satoko Kawauchi, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, Mitsuhiro Terakawa

    研究成果: Conference contribution

    2 被引用数 (Scopus)

    抄録

    Control of wound sepsis is an important challenge in traumatology. However, increase in the drug-resistant bacteria makes this challenge considerably difficult in recent years. In this study, we attempted to control burn wound sepsis in rats by photodynamic treatment, which has been reported to be effective against some drug-resistant bacteria. A 20% TBSA (total body surface area) full-thickness burn was made in rat dorsal skin, and five days after injury, a suspension of P. aeruginosa was applied to the wound surface. At 30 min after infection, a methylene blue (MB) solution was applied to the wound surface; 5 min afterwards, the wound was illuminated with a 665-nm light emitting diode (LED) array for 10 min. This treatment (application of MB and illumination) was repeated 3 times successively. The averaged light intensity on the wound surface was 3.3 mW/cm 2, the corresponding total light dose being 5.9 J/cm 2. One week after injury, the numbers of bacteria in the blood and liver were counted by colony forming assay. In the liver, the number of bacteria of the treated group was significantly lower than that of the sham control group without photodynamic treatment. In the blood, no bacteria were detected in the treated group, while a certain amount of bacteria was detected in the control group. These results demonstrate the efficacy of MB-mediated PDT with a red LED array to control burn wound sepsis.

    本文言語English
    ホスト出版物のタイトルOptical Methods for Tumor Treatment and Detection
    ホスト出版物のサブタイトルMechanisms and Techniques in Photodynamic Therapy XXI
    DOI
    出版ステータスPublished - 2012 4 9
    イベントOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI - San Francisco, CA, United States
    継続期間: 2012 1 212012 1 22

    出版物シリーズ

    名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
    8210
    ISSN(印刷版)1605-7422

    Other

    OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXI
    CountryUnited States
    CitySan Francisco, CA
    Period12/1/2112/1/22

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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