We prepared the particle monolayer, which could display a hexagonal arrangement pattern of the contact point ranging from nanometer to micrometer scales, by Langmuir-Blodgett method. HUVECs were seeded on the particle monolayer and the cell shapes were observed with a fluorescent microscope. As the pitch of particle monolayers became large, cells became a round shape and cell migration was suppressed. Most cells uniformly adhered onto the non-treated particle monolayer in a few hours at the seeding density of 200,000 cells/cm2, whereas cells on the non-treated PS dish formed spheroid-like aggregates. To make a non-adhesive region, BSA molecules were immobilized onto the particle monolayer by the photolithography. When HUVECs were seeded on the photopatterned particle monolayer, cells tended not to adhere onto the BSA-immobilized region on the particle monolayer, resulting in adhering onto the non-treated region.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
継続期間: 2005 9月 20 → 2005 9月 22
|Other||54th SPSJ Symposium on Macromolecules|
|Period||05/9/20 → 05/9/22|
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