Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: Preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes

Mieko Kashiwagi, Ken Ichi Fuhshuku, Takeshi Sugai

研究成果: Article

20 引用 (Scopus)

抜粋

The reaction conditions towards the preferential action of either nitrile hydratase or amidase in the harvested whole cells of Rhodococcus rhodochrous IFO 15564 were elaborated. The amidase showed higher heat tolerance than the nitrile hydratase and, at 45°C the amidase worked exclusively. DMSO assisted the preferential action of nitrile hydratase, however, at more than 30% (v/v) addition of DMF, the nitrile hydratase activity was completely lost and only amidase worked. A one-pot chemo-enzymatic conversion of aldehydes to amides [(1) aq. NH3, I2, DMSO; (2) Na2S2O 3; (3) harvested cells of R. rhodochrous] was established. Under these reaction conditions, most of the amidase was lost, and the incubation of the firstly formed intermediates, nitriles in aq. NH3 was responsible for the selective inhibition of amidase. The freezing of harvested cells in an exhaustively deionized environment provided a long-term preservable "ready to use" for the organic chemist.

元の言語English
ページ(範囲)249-258
ページ数10
ジャーナルJournal of Molecular Catalysis B: Enzymatic
29
発行部数1-6
DOI
出版物ステータスPublished - 2004 6 21

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

フィンガープリント Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: Preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用