Controlled gene expression in primary Lgr5 organoid cultures

Bon Kyoung Koo, Daniel E. Stange, Toshiro Sato, Wouter Karthaus, Henner F. Farin, Meritxell Huch, Johan H. Van Es, Hans Clevers

研究成果: Article

183 被引用数 (Scopus)

抄録

The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinaseinducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

本文言語English
ページ(範囲)81-83
ページ数3
ジャーナルNature Methods
9
1
DOI
出版ステータスPublished - 2012 1 1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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