Conversion of β-human atrial natriuretic polypeptide into α-human atrial natriuretic polypeptide in human plasma in vitro

Hiroshi Itoh, Kazuwa Nakao, Shozo Shiono, Masashi Mukoyama, Narito Morii, Akira Sugawara, Takayuki Yamada, Yoshihiko Saito, Hiroshi Arai, Yoshikazu Kambayashi, Ken Inouye, Hiroo Imura

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Using synthetic β-human atrial natriuretic polypeptide (β-hANP), an antiparallel dimer of α-hANP, and radioimmunoassay (RIA) for α-ANP which also detects β-hANP, we investigated the disappearance profile and the change in the molecular form of exogenously added β-hANP in human plasma in vitro, compared with those of α-hANP. The ANP-like immunoreactivity (ANP-LI) level in β-hANP-added human plasma exhibited slower disappearance than that in α-hANP-added plasma during the incubation at 37°C. High performance-gel permeation chromatography and reverse phase-high performance liquid chromatography coupled with RIA revealed that β-hANP (6K) was converted into a smaller peptide with an approximate molecular weight of 3K corresponding to α-hANP during the incubation. Amino acid analysis and amino-terminal sequencing confirmed that the converted peptide from β-hANP in human plasma is authentic α-hANP. The demonstrated conversion of β-hANP into α-hANP in human plasma could be relevant to the in vivo natriuretic and diuretic actions with slower onset and longer duration of this unique peptide.

本文言語English
ページ(範囲)560-569
ページ数10
ジャーナルBiochemical and Biophysical Research Communications
143
2
DOI
出版ステータスPublished - 1987 3月 13
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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