Conversion of primordial germ cells to pluripotent stem cells: Methods for cell tracking and culture conditions

Go Nagamatsu, Toshio Suda

研究成果: Chapter

4 被引用数 (Scopus)

抄録

Primordial germ cells (PGCs) are unipotent cells committed to germ lineage: PGCs can only differentiate into gametes in vivo. However, upon fertilization, germ cells acquire the capacity to differentiate into all cell types in the body, including germ cells. Therefore, germ cells are thought to have the potential for pluripotency. PGCs can convert to pluripotent stem cells in vitro when cultured under specific conditions that include bFGF, LIF, and the membrane-bound form of SCF (mSCF). Here, the culture conditions which efficiently convert PGCs to pluripotent embryonic germ (EG) cells are described, as well as methods used for identifying pluripotent candidate cells during culture.

本文言語English
ホスト出版物のタイトルImaging and Tracking Stem Cells
ホスト出版物のサブタイトルMethods and Protocols
ページ49-56
ページ数8
DOI
出版ステータスPublished - 2013 9 4

出版物シリーズ

名前Methods in Molecular Biology
1052
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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