Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development

Yoshiaki Kubota, Yuichi Oike, Shinya Satoh, Yoko Tabata, Yuichi Niikura, Toru Morisada, Masaki Akao, Takashi Urano, Yasuhiro Ito, Takeshi Miyamoto, Norihiro Nagai, Gou Young Koh, Sumiko Watanabe, Toshio Suda

研究成果: Article

70 引用 (Scopus)

抄録

Angiopoietin-like protein (Angptl) 1 and Angptl2, which are considered orphan ligands, are highly homologous, particularly in the fibrinogen-like domain containing the putative receptor binding site. This similarity suggests potentially cooperative functions between the two proteins. In this report, the function of Angptl1 and Angptl2 is analyzed by using morpholino antisense technology in zebrafish. Knockdown of both Angptl1 and Angptl2 produced severe vascular defects due to increased apoptosis of endothelial cells at the sprouting stage. In vitro studies showed that Angptl1 and Angptl2 have antiapoptotic activities through the phosphatidylinositol 3-kinase Akt pathway, and coinjection of constitutively active Akt/protein kinase B mRNA rescued impaired vascular development seen in double knockdown embryos. These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities.

元の言語English
ページ(範囲)13502-13507
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
102
発行部数38
DOI
出版物ステータスPublished - 2005 9 20

Fingerprint

Angiopoietins
Phosphatidylinositol 3-Kinase
Blood Vessels
Endothelial Cells
Morpholinos
Proto-Oncogene Proteins c-akt
Zebrafish
Fibrinogen
Proteins
Embryonic Structures
Binding Sites
Apoptosis
Ligands
Technology
Messenger RNA
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • General

これを引用

Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development. / Kubota, Yoshiaki; Oike, Yuichi; Satoh, Shinya; Tabata, Yoko; Niikura, Yuichi; Morisada, Toru; Akao, Masaki; Urano, Takashi; Ito, Yasuhiro; Miyamoto, Takeshi; Nagai, Norihiro; Koh, Gou Young; Watanabe, Sumiko; Suda, Toshio.

:: Proceedings of the National Academy of Sciences of the United States of America, 巻 102, 番号 38, 20.09.2005, p. 13502-13507.

研究成果: Article

Kubota, Y, Oike, Y, Satoh, S, Tabata, Y, Niikura, Y, Morisada, T, Akao, M, Urano, T, Ito, Y, Miyamoto, T, Nagai, N, Koh, GY, Watanabe, S & Suda, T 2005, 'Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development', Proceedings of the National Academy of Sciences of the United States of America, 巻. 102, 番号 38, pp. 13502-13507. https://doi.org/10.1073/pnas.0501902102
Kubota, Yoshiaki ; Oike, Yuichi ; Satoh, Shinya ; Tabata, Yoko ; Niikura, Yuichi ; Morisada, Toru ; Akao, Masaki ; Urano, Takashi ; Ito, Yasuhiro ; Miyamoto, Takeshi ; Nagai, Norihiro ; Koh, Gou Young ; Watanabe, Sumiko ; Suda, Toshio. / Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development. :: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; 巻 102, 番号 38. pp. 13502-13507.
@article{8d8ba17ceb344acea06e30fef9238207,
title = "Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development",
abstract = "Angiopoietin-like protein (Angptl) 1 and Angptl2, which are considered orphan ligands, are highly homologous, particularly in the fibrinogen-like domain containing the putative receptor binding site. This similarity suggests potentially cooperative functions between the two proteins. In this report, the function of Angptl1 and Angptl2 is analyzed by using morpholino antisense technology in zebrafish. Knockdown of both Angptl1 and Angptl2 produced severe vascular defects due to increased apoptosis of endothelial cells at the sprouting stage. In vitro studies showed that Angptl1 and Angptl2 have antiapoptotic activities through the phosphatidylinositol 3-kinase Akt pathway, and coinjection of constitutively active Akt/protein kinase B mRNA rescued impaired vascular development seen in double knockdown embryos. These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities.",
keywords = "Akt, Angiogenesis, Apoptosis, Morpholino, Phosphatidylinositol 3-kinase",
author = "Yoshiaki Kubota and Yuichi Oike and Shinya Satoh and Yoko Tabata and Yuichi Niikura and Toru Morisada and Masaki Akao and Takashi Urano and Yasuhiro Ito and Takeshi Miyamoto and Norihiro Nagai and Koh, {Gou Young} and Sumiko Watanabe and Toshio Suda",
year = "2005",
month = "9",
day = "20",
doi = "10.1073/pnas.0501902102",
language = "English",
volume = "102",
pages = "13502--13507",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "38",

}

TY - JOUR

T1 - Cooperative interaction of Angiopoietin-like proteins 1 and 2 in vascular development

AU - Kubota, Yoshiaki

AU - Oike, Yuichi

AU - Satoh, Shinya

AU - Tabata, Yoko

AU - Niikura, Yuichi

AU - Morisada, Toru

AU - Akao, Masaki

AU - Urano, Takashi

AU - Ito, Yasuhiro

AU - Miyamoto, Takeshi

AU - Nagai, Norihiro

AU - Koh, Gou Young

AU - Watanabe, Sumiko

AU - Suda, Toshio

PY - 2005/9/20

Y1 - 2005/9/20

N2 - Angiopoietin-like protein (Angptl) 1 and Angptl2, which are considered orphan ligands, are highly homologous, particularly in the fibrinogen-like domain containing the putative receptor binding site. This similarity suggests potentially cooperative functions between the two proteins. In this report, the function of Angptl1 and Angptl2 is analyzed by using morpholino antisense technology in zebrafish. Knockdown of both Angptl1 and Angptl2 produced severe vascular defects due to increased apoptosis of endothelial cells at the sprouting stage. In vitro studies showed that Angptl1 and Angptl2 have antiapoptotic activities through the phosphatidylinositol 3-kinase Akt pathway, and coinjection of constitutively active Akt/protein kinase B mRNA rescued impaired vascular development seen in double knockdown embryos. These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities.

AB - Angiopoietin-like protein (Angptl) 1 and Angptl2, which are considered orphan ligands, are highly homologous, particularly in the fibrinogen-like domain containing the putative receptor binding site. This similarity suggests potentially cooperative functions between the two proteins. In this report, the function of Angptl1 and Angptl2 is analyzed by using morpholino antisense technology in zebrafish. Knockdown of both Angptl1 and Angptl2 produced severe vascular defects due to increased apoptosis of endothelial cells at the sprouting stage. In vitro studies showed that Angptl1 and Angptl2 have antiapoptotic activities through the phosphatidylinositol 3-kinase Akt pathway, and coinjection of constitutively active Akt/protein kinase B mRNA rescued impaired vascular development seen in double knockdown embryos. These results provide a physiological demonstration of the cooperative interaction of Angptl1 and Angptl2 in endothelial cells through phosphatidylinositol 3-kinase/Akt mediated antiapoptotic activities.

KW - Akt

KW - Angiogenesis

KW - Apoptosis

KW - Morpholino

KW - Phosphatidylinositol 3-kinase

UR - http://www.scopus.com/inward/record.url?scp=26444496162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26444496162&partnerID=8YFLogxK

U2 - 10.1073/pnas.0501902102

DO - 10.1073/pnas.0501902102

M3 - Article

C2 - 16174743

AN - SCOPUS:26444496162

VL - 102

SP - 13502

EP - 13507

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 38

ER -