Correlation between quantitative imaging and behavior in unilaterally 6-OHDA-lesioned rats

Motoki Inaji, Takashi Okauchi, Kiyoshi Ando, Jun Maeda, Yuji Nagai, Takahito Yoshizaki, Hideyuki Okano, Tadashi Nariai, Kikuo Ohno, Shigeru Obayashi, Makoto Higuchi, Tetsuya Suhara

研究成果: Article査読

44 被引用数 (Scopus)

抄録

We evaluated correlation between neurochemical and functional alterations of the nigrostriatal dopaminergic system in rat brains lesioned with 6-hydroxydopamine (6-OHDA), that model hemi-Parkinson's disease (PD), by using three different quantitative in vivo and in vitro methods. Rats unilaterally lesioned with different doses of 6-OHDA underwent two types of in vivo experiments: (1) a rotational behavioral study with methamphetamine (MAP) or apomorphine (APO); and (2) a positron emission tomography (PET) study with [11C]PE2I (radioligand for dopamine transporters) or [ 11C]raclopride (radioligand for dopamine D2 receptors). An in vitro autoradiographic study with the same radioligands was also conducted. The number of rotations after the MAP or APO injection increased with increased doses of 6-OHDA. The in vitro and in vivo binding of [11C]PE2I dose-dependently decreased in response to the 6-OHDA injections, while that of [11C]raclopride dose-dependently increased. There was a significant negative hyperbolic correlation between the number of rotations after MAP injection and the binding of [11C]PE2I. In contrast, there was a significant positive linear correlation between the number of rotations after APO injections and the binding of [11C]raclopride. These results robustly reveal a molecular pharmacological basis of parkinsonian symptoms in animal models of PD, and indicate the utility and validity of in vivo PET measurements in assessing pre- and post-synaptic dopaminergic functions.

本文言語English
ページ(範囲)136-145
ページ数10
ジャーナルBrain Research
1064
1-2
DOI
出版ステータスPublished - 2005 12 7

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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