Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H

Mitsuhiro Itaya, Robert J. Crouch

研究成果: Article査読

16 被引用数 (Scopus)

抄録

The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].

本文言語English
ページ(範囲)433-437
ページ数5
ジャーナルMGG Molecular & General Genetics
227
3
DOI
出版ステータスPublished - 1991 7月 1

ASJC Scopus subject areas

  • 遺伝学

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