cortex

Rei Murata, Yuki Takada, Hiroyuki Takuwa, Hiroshi Kawaguchi, Hiroshi Ito, Iwao Kanno, Naotomo Tottori, Yukio Yamada, Yutaka Tomita, Yoshiaki Itoh, Norihiro Suzuki, Katsuya Yamada, Kazuto Masamoto

研究成果: Article査読

抄録

The present study examined glucose transfer in the cellular scale of mouse brain microvasculature in vivo using two-photon microscopy and fl uorescent glucose analogue (2-NBDG). The 2-NBDG was intravenously injected (0.04 mL/ min) in the anesthetized Tie2-GFP mice in which the vascular endothelium expressed fl uorescent protein. Time-lapse imaging was conducted on the cortical parenchyma, while the time–intensity change of the injected 2-NBDG was analysed in respective vascular compartments (artery, capillary, and vein). We observed that 2-NBDG signal increased monotonically in the vasculature during the period of the injection, and rapidly declined following its cessation. In tissue compartment, however, the signal intensity gradually increased even after cessation of the injection. Spatiotemporal analysis of the 2-NBDG intensity over the cross-sections of the vessels further showed distinct change of the 2-NBDG intensity across the vessel wall (endothelium), which may represents a regulation site of tissue glucose infl ux.

本文言語English
ページ(範囲)241-246
ページ数6
ジャーナルAdvances in experimental medicine and biology
812
DOI
出版ステータスPublished - 2014
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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