CrkL directs ASAP1 to peripheral focal adhesions

Atsushi Oda, Ikuo Wada, Koichi Miura, Katsuya Okawa, Toshihiko Kadoya, Takashi Kato, Hiroshi Nishihara, Masae Maeda, Shinya Tanaka, Kazuo Nagashima, Chiaki Nishitani, Kazuhiko Matsuno, Masaho Ishino, Laura M. Machesky, Hiroyoshi Fujita, Paul Randazzo

研究成果: Article

31 引用 (Scopus)

抄録

Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP), ASAP1, as a CrkL-binding protein. In spreading platelets, most endogenous ASAP1 is localized at peripheral focal adhesions. To determine the physiologic significance of the CrkL-ASAP1 association, we overexpressed CrkL, ASAP1, or both in combination in COS7 cells. Unlike endogenous ASAP1 in platelets, overexpressed ASAP1 showed diffuse cytoplasmic distribution. However, when co-expressed with wild-type CrkL, both endogenous and expressed ASAP1 accumulated at CrkL-induced focal adhesions. An SH2-mutated CrkL, which cannot localize at focal adhesions, failed to recruit ASAP1 into focal adhesions. Thus, CrkL appears to be a lynchpin between ASAP1 and peripheral focal adhesions.

元の言語English
ページ(範囲)6456-6460
ページ数5
ジャーナルJournal of Biological Chemistry
278
発行部数8
DOI
出版物ステータスPublished - 2003 2 21
外部発表Yes

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Focal Adhesions
Platelets
Adhesion
Blood Platelets
ADP-Ribosylation Factors
GTPase-Activating Proteins
src Homology Domains
Mass spectrometry
Assays
Mass Spectrometry
Carrier Proteins
Association reactions
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Oda, A., Wada, I., Miura, K., Okawa, K., Kadoya, T., Kato, T., ... Randazzo, P. (2003). CrkL directs ASAP1 to peripheral focal adhesions. Journal of Biological Chemistry, 278(8), 6456-6460. https://doi.org/10.1074/jbc.M210817200

CrkL directs ASAP1 to peripheral focal adhesions. / Oda, Atsushi; Wada, Ikuo; Miura, Koichi; Okawa, Katsuya; Kadoya, Toshihiko; Kato, Takashi; Nishihara, Hiroshi; Maeda, Masae; Tanaka, Shinya; Nagashima, Kazuo; Nishitani, Chiaki; Matsuno, Kazuhiko; Ishino, Masaho; Machesky, Laura M.; Fujita, Hiroyoshi; Randazzo, Paul.

:: Journal of Biological Chemistry, 巻 278, 番号 8, 21.02.2003, p. 6456-6460.

研究成果: Article

Oda, A, Wada, I, Miura, K, Okawa, K, Kadoya, T, Kato, T, Nishihara, H, Maeda, M, Tanaka, S, Nagashima, K, Nishitani, C, Matsuno, K, Ishino, M, Machesky, LM, Fujita, H & Randazzo, P 2003, 'CrkL directs ASAP1 to peripheral focal adhesions', Journal of Biological Chemistry, 巻. 278, 番号 8, pp. 6456-6460. https://doi.org/10.1074/jbc.M210817200
Oda A, Wada I, Miura K, Okawa K, Kadoya T, Kato T その他. CrkL directs ASAP1 to peripheral focal adhesions. Journal of Biological Chemistry. 2003 2 21;278(8):6456-6460. https://doi.org/10.1074/jbc.M210817200
Oda, Atsushi ; Wada, Ikuo ; Miura, Koichi ; Okawa, Katsuya ; Kadoya, Toshihiko ; Kato, Takashi ; Nishihara, Hiroshi ; Maeda, Masae ; Tanaka, Shinya ; Nagashima, Kazuo ; Nishitani, Chiaki ; Matsuno, Kazuhiko ; Ishino, Masaho ; Machesky, Laura M. ; Fujita, Hiroyoshi ; Randazzo, Paul. / CrkL directs ASAP1 to peripheral focal adhesions. :: Journal of Biological Chemistry. 2003 ; 巻 278, 番号 8. pp. 6456-6460.
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abstract = "Searching for proteins in platelets that can interact with the N-terminal SH3 domain of CrkL (using a combination of a pull-down assay followed by mass spectrometry), we have found that human platelets express an ADP-ribosylation factor (Arf)-specific GTPase-activating protein (GAP), ASAP1, as a CrkL-binding protein. In spreading platelets, most endogenous ASAP1 is localized at peripheral focal adhesions. To determine the physiologic significance of the CrkL-ASAP1 association, we overexpressed CrkL, ASAP1, or both in combination in COS7 cells. Unlike endogenous ASAP1 in platelets, overexpressed ASAP1 showed diffuse cytoplasmic distribution. However, when co-expressed with wild-type CrkL, both endogenous and expressed ASAP1 accumulated at CrkL-induced focal adhesions. An SH2-mutated CrkL, which cannot localize at focal adhesions, failed to recruit ASAP1 into focal adhesions. Thus, CrkL appears to be a lynchpin between ASAP1 and peripheral focal adhesions.",
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AU - Oda, Atsushi

AU - Wada, Ikuo

AU - Miura, Koichi

AU - Okawa, Katsuya

AU - Kadoya, Toshihiko

AU - Kato, Takashi

AU - Nishihara, Hiroshi

AU - Maeda, Masae

AU - Tanaka, Shinya

AU - Nagashima, Kazuo

AU - Nishitani, Chiaki

AU - Matsuno, Kazuhiko

AU - Ishino, Masaho

AU - Machesky, Laura M.

AU - Fujita, Hiroyoshi

AU - Randazzo, Paul

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