Cross-saturation and transferred cross-saturation experiments

Takumi Ueda, Koh Takeuchi, Noritaka Nishida, Pavlos Stampoulis, Yutaka Kofuku, Masanori Osawa, Ichio Shimada

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Structural analyses of protein-protein interactions are required to reveal their functional mechanisms, and accurate protein-protein complex models, based on experimental results, are the starting points for drug development. In addition, structural information about proteins under physiologically relevant conditions is crucially important for understanding biological events. However, for proteins such as those embedded in lipid bilayers and transiently complexed with their effectors under physiological conditions, structural analyses by conventional methods are generally difficult, due to their large molecular weights and inhomogeneity. We have developed the cross-saturation (CS) method, which is an nuclear magnetic resonance measurement technique for the precise identification of the interfaces of protein-protein complexes. In addition, we have developed an extended version of the CS method, termed transferred cross-saturation (TCS), which enables the identification of the residues of protein ligands in close proximity to huge (>150 kDa) and heterogeneous complexes under fast exchange conditions (>0.1 s-1). Here, we discuss the outline, basic theory, and practical considerations of the CS and TCS methods. In addition, we will review the recent progress in the construction of models of protein-protein complexes, based on CS and TCS experiments, and applications of TCS to in situ analyses of biologically and medically important proteins in physiologically relevant states.

本文言語English
ページ(範囲)143-187
ページ数45
ジャーナルQuarterly Reviews of Biophysics
47
2
DOI
出版ステータスPublished - 2014 5
外部発表はい

ASJC Scopus subject areas

  • Biophysics

フィンガープリント 「Cross-saturation and transferred cross-saturation experiments」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル