Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus

Masayoshi Nakasako, Makoto Kimura, Isamu Yamaguchi

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Blasticidin S deaminase from Aspergillus terreus was crystallized with polyethylene glycol 8000. Two types of crystals were grown under the same crystallization conditions. One type grew as thin plates, while the other had a rhombic shape. The rhombic shaped crystal was suitable for high-resolution crystal structure analysis. Precession photographs and diffraction data showed that the crystal belonged to orthorhombic space group P212121, with unit-cell dimensions a = 70.33, b = 146.56 and c = 56.48 Å. The calculated V(m) value was acceptable when a tetramer of the enzyme was contained in an asymmetric unit. Preliminary diffraction data were collected to a resolution of 2.0 Å with good statistics.

本文言語English
ページ(範囲)547-548
ページ数2
ジャーナルActa Crystallographica Section D: Biological Crystallography
55
2
DOI
出版ステータスPublished - 1999 2 1
外部発表はい

ASJC Scopus subject areas

  • 構造生物学

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