Crystallographic characterization by X-ray diffraction of the M-intermediate from the photo-cycle of bacteriorhodopsin at room temperature

Masayoshi Nakasako, Mikio Kataoka, Yoshiyuki Amemiya, Fumio Tokunaga

研究成果: Article査読

94 被引用数 (Scopus)

抄録

The structure of the M-intermediate appearing in the photo-cycle of bacteriorhodopsin was studied with X-ray diffraction techniques at room temperature. The lifetime of the M-intermediate was prolonged by treatment with an arginine solution at alkaline pH (Nakasako et al.,FEBS Lett. 254, 211-214). The diffraction profile of membranes which had accumulated the M-intermediate had small but significant differences in the intensities of Bragg reflections and the lattice constant in comparison with that of membranes having trans-bacteriorhodopsin. Diffraction intensities were carefully evaluated and the structural changes during the formation of the intermediate were evaluated with difference Fourier analysis. We could find structural changes around helices G and B.

本文言語English
ページ(範囲)73-75
ページ数3
ジャーナルFEBS Letters
292
1-2
DOI
出版ステータスPublished - 1991 11月 4
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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