Culturing intestinal stem cells: Applications for colorectal cancer research

Masayuki Fujii, Toshiro Sato

研究成果: Short survey

4 引用 (Scopus)

抄録

Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of intestinal stem cell self-renewal, leading to the development of novel human intestinal organoid culture system. Intestinal organoid culture enabled to expand normal or tumor epithelial cells in vitro retaining their stem cell self-renewal and multiple differentiation. Gene manipulation of these cultured cells may provide an attractive tool for investigating genetic events involved in colorectal carcinogenesis.

元の言語English
記事番号169
ジャーナルFrontiers in Genetics
5
発行部数JUN
DOI
出版物ステータスPublished - 2014

Fingerprint

Organoids
Colorectal Neoplasms
Stem Cells
Genetic Models
Human Development
Intestinal Mucosa
Research
Genes
Cultured Cells
Carcinogenesis
Theoretical Models
Epithelial Cells
Technology
Cell Line
Neoplasms
Cell Self Renewal
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Molecular Medicine
  • Genetics(clinical)

これを引用

Culturing intestinal stem cells : Applications for colorectal cancer research. / Fujii, Masayuki; Sato, Toshiro.

:: Frontiers in Genetics, 巻 5, 番号 JUN, 169, 2014.

研究成果: Short survey

@article{a1f6d257cbb947c0817fe2285bfa1115,
title = "Culturing intestinal stem cells: Applications for colorectal cancer research",
abstract = "Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of intestinal stem cell self-renewal, leading to the development of novel human intestinal organoid culture system. Intestinal organoid culture enabled to expand normal or tumor epithelial cells in vitro retaining their stem cell self-renewal and multiple differentiation. Gene manipulation of these cultured cells may provide an attractive tool for investigating genetic events involved in colorectal carcinogenesis.",
keywords = "Cancer stem cells (CSC), Niche, Organoids, R-spondin, Wnt proteins",
author = "Masayuki Fujii and Toshiro Sato",
year = "2014",
doi = "10.3389/fgene.2014.00169",
language = "English",
volume = "5",
journal = "Frontiers in Genetics",
issn = "1664-8021",
publisher = "Frontiers Media S. A.",
number = "JUN",

}

TY - JOUR

T1 - Culturing intestinal stem cells

T2 - Applications for colorectal cancer research

AU - Fujii, Masayuki

AU - Sato, Toshiro

PY - 2014

Y1 - 2014

N2 - Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of intestinal stem cell self-renewal, leading to the development of novel human intestinal organoid culture system. Intestinal organoid culture enabled to expand normal or tumor epithelial cells in vitro retaining their stem cell self-renewal and multiple differentiation. Gene manipulation of these cultured cells may provide an attractive tool for investigating genetic events involved in colorectal carcinogenesis.

AB - Recent advance of sequencing technology has revealed genetic alterations in colorectal cancer (CRC). The biological function of recurrently mutated genes has been intensively investigated through mouse genetic models and CRC cell lines. Although these experimental models may not fully reflect biological traits of human intestinal epithelium, they provided insights into the understanding of intestinal stem cell self-renewal, leading to the development of novel human intestinal organoid culture system. Intestinal organoid culture enabled to expand normal or tumor epithelial cells in vitro retaining their stem cell self-renewal and multiple differentiation. Gene manipulation of these cultured cells may provide an attractive tool for investigating genetic events involved in colorectal carcinogenesis.

KW - Cancer stem cells (CSC)

KW - Niche

KW - Organoids

KW - R-spondin

KW - Wnt proteins

UR - http://www.scopus.com/inward/record.url?scp=84906283594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906283594&partnerID=8YFLogxK

U2 - 10.3389/fgene.2014.00169

DO - 10.3389/fgene.2014.00169

M3 - Short survey

AN - SCOPUS:84906283594

VL - 5

JO - Frontiers in Genetics

JF - Frontiers in Genetics

SN - 1664-8021

IS - JUN

M1 - 169

ER -