Cyclic AMP-dependent PKA phosphorylates starfish sperm proteins during acrosome reaction

M. Sadiqul Islam, Motonori Hoshi, Midori Matsumoto

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The induction of acrosome reaction (AR) happens when starfish spermatozoa encounter the egg jelly (EJ). This complex process involves different signal transduction pathways, such as elevation of cAMP and the activation of protein kinase A (PKA). The specific inhibitors of PKA (H89 and KT5720) have been shown to inhibit the EJ-induced Ca2+ elevation and AR. By using a Phospho-Ser/Thr PKA substrate antibody, we have detected an increased phosphorylation of 150, 200 and 220-kDa protein bands when starfish spermatozoa treated with EJ. The specific PKA inhibitors effectively inhibit phosphorylation of these proteins, suggesting an involvement of PKA on EJ-induced AR.

本文言語English
ページ(範囲)109-121
ページ数13
ジャーナルCentral European Journal of Biology
2
1
DOI
出版ステータスPublished - 2007 3

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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