Cysteine proteinases and cystatin C in bronchoalveolar lavage fluid from subjects with subclinical emphysema

K. Takeyabu, T. Betsuyaku, M. Nishimura, A. Yoshioka, M. Tanino, K. Miyamoto, Y. Kawakami

研究成果: Article査読

62 被引用数 (Scopus)

抄録

This study examined the role of cysteine proteinases and their inhibitor in the development of emphysema in comparison with neutrophil elastase (NE) complexed with α1-protease inhibitor (NE-α1PI), which was previously demonstrated to be increased in bronchoalveolar lavage (BAL) fluid from subjects with subclinical emphysema. Eight nonsmokers and 31 current smokers with (n=17) and without (n=14) emphysema, as evidenced by lung computed tomographic scans, were studied. The concentrations of immunologically detected cathepsin L and cystatin C, but not cathepsin B, were significantly increased in BAL fluid from the smokers with emphysema compared with those without emphysema, although the activity of cathepsin L, measured using a synthetic substrate and cathepsin L released from cultured alveolar macrophages at 24 h, did not show any significant difference between the two groups. When comparison was made only for the subjects aged <60 yrs, the difference between the two groups disappeared for cathepsin L, but remained for NEα1-PI. There was no significant correlation between the level of cathepsin L and that of NE-α1-PI in BAL fluid from the subjects with emphysema. In conclusion, increased levels of cathepsin L and cystatin C were demonstrated in bronchoalveolar lavage fluid from subjects with subclinical emphysema. However, the roles of cathepsin L and neutrophil elastase in the development of emphysema may vary between subjects and between the young and the old.

本文言語English
ページ(範囲)1033-1039
ページ数7
ジャーナルEuropean Respiratory Journal
12
5
DOI
出版ステータスPublished - 1998 11月

ASJC Scopus subject areas

  • 呼吸器内科

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