Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines

Fusako Nagai, Yasuko Hiyoshi, Kumiko Sugimachi, Hiro Omi Tamura

研究成果: Article査読

70 被引用数 (Scopus)

抄録

To explore the physiological roles of cytochrome P450 (CYP) in peripheral blood cells, we examined which isoforms of CYP families were expressed in human myeloid leukemia cell lines (U937, HL-60 and K562) and lymphoid cell lines (BALL-1, MOLT-4 and Jurkat) by RT-PCR. We observed relatively high expression of CYP1A1, CYP1B1, CYP2A6, CYP2A7, CYP2D6, and CYP2E1 in all cell types, but CYP2A13 and CYP2C9 expression was not detected. Expressions of aryl hydrocarbon (Ah) receptor and Ah receptor nuclear translocator (ARNT), which mediate induction of the CYP1 family, were also detected in all cell types. Cell-type specific expression of CYP3A4 and CYP3A5 was observed in MOLT-4 and K562 cells. Weak, but significant, expression of CYP3A7 was detected in K562 cells. The profile of CYP expression in the culture cells reported here provides information that furthers our understanding of the physiological roles of CYP enzymes in human blood cells.

本文言語English
ページ(範囲)383-385
ページ数3
ジャーナルBiological and Pharmaceutical Bulletin
25
3
DOI
出版ステータスPublished - 2002 3

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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