Cytostatic effect of inostamycin, an inhibitor of cytidine 5′-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): Inositol transferase, on oral squamous cell carcinoma cell lines

Yuh Baba, Mamoru Tsukuda, Izumi Mochimatsu, Shigeru Furukawa, Hiroko Kagata, Yoji Nagashima, Shinri Koshika, Masaya Imoto, Yasumasa Kato

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Inostamycin, which was recently isolated from Streptomyces sp. MH816-AF15 as an inhibitor of cytidine 5′-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): inositol transferase, caused a G1-phase accumulation in the cell cycle of small cell lung carcinomas. To investigate whether the cytostatic effect of inostamycin is restricted to lung carcinoma cell lines or applicable to other type of cells, we tested five oral squamous cell carcinoma (SCC) cell lines. Cell growth was suppressed in 62.5-125 ng/ml inostamycin in the culture medium in all oral cancer cell lines tested, with non-viable cells being < 1%, indicating inostamycin is cytostatic on SCC cell lines. Decrease in cyclin D1 mRNA and protein expression due to the inostamycin treatment was accompanied by suppression of phosphorylated retinoblastoma susceptibility gene product (pRB-P) levels. Moreover, flow cytometric analysis showed that inostamycin induced an increase in G1/G0 cells (1.2-3.2 fold) over 24 h. These results suggest that inostamycin is a useful agent for tumour dormant cytostatic therapy for oral SCC.

本文言語English
ページ(範囲)613-620
ページ数8
ジャーナルCell Biology International
25
7
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 細胞生物学

フィンガープリント

「Cytostatic effect of inostamycin, an inhibitor of cytidine 5′-diphosphate 1,2-diacyl-sn-glycerol (CDP-DG): Inositol transferase, on oral squamous cell carcinoma cell lines」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル