TY - JOUR
T1 - Cytotoxicity of estradiol, equilin, equilenin, and their derivatives on Chinese hamster V79 cells
AU - Oda, Taiko
AU - Ikeda, Noriko
AU - Nakashima, Shoko
AU - Sato, Yoshihiro
PY - 2002/2/18
Y1 - 2002/2/18
N2 - Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.
AB - Recently, we investigated the inhibitory effects of 17β-estradiol and diethylstilbestrol on microtubule assembly, cytotoxicity, and aneuploidy in V79 cells. The present study analyzes the effects of equilin and equilenin (amongst the natural estrogens originally isolated from the urine of pregnant horses) and their related compounds, on the relative plating efficiency of Chinese hamster V79 cells. The results showed that a hydroxyl group on 17-C and a methoxyl group on 3-C of the estrogen skeleton were important for cytotoxicity. Of the various compounds analyzed, 2-methoxyestradiol had the strongest cytotoxicity, suggesting also the importance of a methoxyl group on 2-C.
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U2 - 10.1081/DCT-100108473
DO - 10.1081/DCT-100108473
M3 - Article
C2 - 11850971
AN - SCOPUS:0036166267
VL - 25
SP - 75
EP - 82
JO - Drug and Chemical Toxicology
JF - Drug and Chemical Toxicology
SN - 0148-0545
IS - 1
ER -
