Defective smooth muscle development in qkl-deficient mice

Zhenghua Li, Nobuyuki Takakura, Yuichi Oike, Takanobu Imanaka, Kimi Araki, Toshio Suda, Tadashi Kaname, Tatsuya Kondo, Kuniya Abe, Ken Ichi Yamamura

研究成果: Article査読

53 被引用数 (Scopus)

抄録

The qkl gene encodes an RNA binding protein which was identified as a candidate for the classical neurologic mutation, qkv. Although qkl is involved in glial cell differentiation in mice, qkl homologues in other species play important roles in various developmental processes. Here, we show a novel function of qkl in smooth muscle cell differentiation during embryonic blood vessel formation, qkl null embryos died between embryonic day 9.5 and 10.5. Embryonic day 9.5 qkl null embryos showed a lack of large vitelline vessels in the yolk sacs, kinky neural tubes, pericardial effusion, open neural tubes and incomplete embryonic turning. Using X-gal and immunohistochemical staining, qkl is first shown to be expressed in endothelial cells and smooth muscle cells. Analyses of qkl null embryos in vivo and in vitro revealed that the vitelline artery was too thin to connect properly to the yolk sac, thereby preventing remodeling of the yolk sac vasculature, and that the vitelline vessel was deficient in smooth muscle cells. Addition of QKI and platelet-endothelial cell adhesion molecule-1 positive cells to an in vitro para-aortic splanchnopleural culture of qkl null embryos rescued the vascular remodeling deficit. These data suggest that QKI protein has a critical regulatory role in smooth muscle cell development, and that smooth muscle cells play an important role in inducing vascular remodeling.

本文言語English
ページ(範囲)449-462
ページ数14
ジャーナルDevelopment Growth and Differentiation
45
5-6
DOI
出版ステータスPublished - 2003 10

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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