Deficiency of inducible nitric oxide synthase exacerbates hepatic fibrosis in mice fed high-fat diet

Yi Chen, Shigenari Hozawa, Sadaaki Sawamura, Shinkichi Sato, Naoto Fukuyama, Chizuko Tsuji, Tetsuya Mine, Yasunori Okada, Ryuzaburo Tanino, Yoichi Ogushi, Hiroe Nakazawa

研究成果: Article査読

42 被引用数 (Scopus)

抄録

The role of inducible nitric oxide synthase (iNOS) in the progression of fibrosis during nonalcoholic steatohepatitis remains to be elucidated. This study examined the role of iNOS in the progression of fibrosis during steatohepatitis by comparing iNOS knockout (iNOS -/-) and wild-type (iNOS +/+) mice that were fed a high-fat diet. Severe fatty metamorphosis developed in the liver of iNOS +/+ and iNOS -/- mice. Fibrotic changes were marked in iNOS -/- mice. Gelatin zymography showed that pro MMP-2 and pro MMP-9 protein expressions were more highly induced in iNOS +/+ mice than in iNOS -/- mice. Active forms of MMP-2 and MMP-9 were clearly present only in the liver tissue of iNOS +/+ mice. In situ zymography showed strong gelatinolytic activities in the liver tissue of iNOS +/+ mice, but only spotty activity in iNOS -/-mice. iNOS may attenuate the progression of liver fibrosis in steatohepatitis, in part by inducing MMP-2 and MMP-9 expression and augmenting their activity.

本文言語English
ページ(範囲)45-51
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
326
1
DOI
出版ステータスPublished - 2004 12 31
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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