TY - JOUR
T1 - Degradation of Extracellular Matrix by Matrix Metalloproteinase 2 Is Essential for the Establishment of the Blood-Brain Barrier in Drosophila
AU - Kanda, Hiroshi
AU - Shimamura, Rieko
AU - Koizumi-Kitajima, Michiko
AU - Okano, Hideyuki
PY - 2019/6/28
Y1 - 2019/6/28
N2 - The blood-brain barrier (BBB) is an essential system that isolates the central nervous system from the internal environment. Increasing evidence has begun to reveal the molecules that are required for BBB integrity. However, how these components are regulated remains unclear. Here we report that a matrix metalloproteinase, Mmp2, is essential for the establishment of the BBB in Drosophila. In the absence of mmp2, the BBB becomes leaky, which allows the tracer to penetrate the brain. Moreover, the expression pattern of a junctional component, Neuroglian, is altered. We also find that the regulation of the amounts of particular extracellular matrix components is critical for BBB establishment. Furthermore, the process of mesenchymal-epithelial transition of BBB-forming cells is perturbed in the absence of Mmp2. These data indicate that the presence of Mmp(s), which is typically considered to be a risk factor for BBB degradation, is essential for BBB integrity in Drosophila. Biological Sciences; Molecular Biology; Cell Biology
AB - The blood-brain barrier (BBB) is an essential system that isolates the central nervous system from the internal environment. Increasing evidence has begun to reveal the molecules that are required for BBB integrity. However, how these components are regulated remains unclear. Here we report that a matrix metalloproteinase, Mmp2, is essential for the establishment of the BBB in Drosophila. In the absence of mmp2, the BBB becomes leaky, which allows the tracer to penetrate the brain. Moreover, the expression pattern of a junctional component, Neuroglian, is altered. We also find that the regulation of the amounts of particular extracellular matrix components is critical for BBB establishment. Furthermore, the process of mesenchymal-epithelial transition of BBB-forming cells is perturbed in the absence of Mmp2. These data indicate that the presence of Mmp(s), which is typically considered to be a risk factor for BBB degradation, is essential for BBB integrity in Drosophila. Biological Sciences; Molecular Biology; Cell Biology
KW - Biological Sciences
KW - Cell Biology
KW - Molecular Biology
UR - http://www.scopus.com/inward/record.url?scp=85066922175&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066922175&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2019.05.027
DO - 10.1016/j.isci.2019.05.027
M3 - Article
AN - SCOPUS:85066922175
VL - 16
SP - 218
EP - 229
JO - iScience
JF - iScience
SN - 2589-0042
ER -