Degradation of phosphate polymer polyP enhances lactic fermentation in mice

Akihiro Nakamura, Natsuko Kawano, Kei Motomura, Akio Kuroda, Kiyoshi Sekiguchi, Mami Miyado, Woojin Kang, Yoshitaka Miyamoto, Maito Hanai, Maki Iwai, Mitsutoshi Yamada, Toshio Hamatani, Takakazu Saito, Hidekazu Saito, Mamoru Tanaka, Akihiro Umezawa, Kenji Miyado

研究成果: Article

抄録

In bacteria, a polymer of inorganic phosphate (Pi) (inorganic polyphosphate; polyP) is enzymatically produced and consumed as an alternative phosphate donor for adenosine triphosphate (ATP) production to protect against nutrient starvation. In vertebrates, polyP has been dismissed as a “molecular fossil” due to the lack of any known physiological function. Here, we have explored its possible role by producing transgenic (TG) mice widely expressing Saccharomyces cerevisiae exopolyphosphatase 1 (ScPPX1), which catalyzes hydrolytic polyP degradation. TG mice were produced and displayed reduced mitochondrial respiration in muscles. In female TG mice, the blood concentration of lactic acid was enhanced, whereas ATP storage in liver and brain tissues was reduced significantly. Thus, we suggested that the elongation of polyP reduces the intracellular Pi concentration, suppresses anaerobic lactic acid production, and sustains mitochondrial respiration. Our results provide an insight into the physiological role of polyP in mammals, particularly in females.

元の言語English
ページ(範囲)904-914
ページ数11
ジャーナルGenes to Cells
23
発行部数10
DOI
出版物ステータスPublished - 2018 10 1

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Polyps
Fermentation
Polymers
Milk
Phosphates
Transgenic Mice
Lactic Acid
Respiration
Adenosine Triphosphate
Polyphosphates
Starvation
Saccharomyces cerevisiae
Vertebrates
Mammals
Bacteria
Food
Muscles
Liver
Brain

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

これを引用

Nakamura, A., Kawano, N., Motomura, K., Kuroda, A., Sekiguchi, K., Miyado, M., ... Miyado, K. (2018). Degradation of phosphate polymer polyP enhances lactic fermentation in mice. Genes to Cells, 23(10), 904-914. https://doi.org/10.1111/gtc.12639

Degradation of phosphate polymer polyP enhances lactic fermentation in mice. / Nakamura, Akihiro; Kawano, Natsuko; Motomura, Kei; Kuroda, Akio; Sekiguchi, Kiyoshi; Miyado, Mami; Kang, Woojin; Miyamoto, Yoshitaka; Hanai, Maito; Iwai, Maki; Yamada, Mitsutoshi; Hamatani, Toshio; Saito, Takakazu; Saito, Hidekazu; Tanaka, Mamoru; Umezawa, Akihiro; Miyado, Kenji.

:: Genes to Cells, 巻 23, 番号 10, 01.10.2018, p. 904-914.

研究成果: Article

Nakamura, A, Kawano, N, Motomura, K, Kuroda, A, Sekiguchi, K, Miyado, M, Kang, W, Miyamoto, Y, Hanai, M, Iwai, M, Yamada, M, Hamatani, T, Saito, T, Saito, H, Tanaka, M, Umezawa, A & Miyado, K 2018, 'Degradation of phosphate polymer polyP enhances lactic fermentation in mice', Genes to Cells, 巻. 23, 番号 10, pp. 904-914. https://doi.org/10.1111/gtc.12639
Nakamura A, Kawano N, Motomura K, Kuroda A, Sekiguchi K, Miyado M その他. Degradation of phosphate polymer polyP enhances lactic fermentation in mice. Genes to Cells. 2018 10 1;23(10):904-914. https://doi.org/10.1111/gtc.12639
Nakamura, Akihiro ; Kawano, Natsuko ; Motomura, Kei ; Kuroda, Akio ; Sekiguchi, Kiyoshi ; Miyado, Mami ; Kang, Woojin ; Miyamoto, Yoshitaka ; Hanai, Maito ; Iwai, Maki ; Yamada, Mitsutoshi ; Hamatani, Toshio ; Saito, Takakazu ; Saito, Hidekazu ; Tanaka, Mamoru ; Umezawa, Akihiro ; Miyado, Kenji. / Degradation of phosphate polymer polyP enhances lactic fermentation in mice. :: Genes to Cells. 2018 ; 巻 23, 番号 10. pp. 904-914.
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AU - Kang, Woojin

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AU - Hanai, Maito

AU - Iwai, Maki

AU - Yamada, Mitsutoshi

AU - Hamatani, Toshio

AU - Saito, Takakazu

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AU - Umezawa, Akihiro

AU - Miyado, Kenji

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