Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type inositol 1,4,5-trisphosphate receptor gene

Yoshiyuki Konishi, Yasushi Kobayashi, Toshihiko Kishimoto, Yasutaka Makino, Atsushi Miyawaki, Teiichi Furuichi, Hideyuki Okano, Katsuhiko Mikoshiba, Taka Aki Tamura

研究成果: Article査読

14 被引用数 (Scopus)

抄録

The type 1 inositol 1,4,5-trisphosphate receptor (IP3R1) is expressed abundantly in the CNS, such as in cerebellar Purkinje cells and the hippocampus. We established a tissue-specific cell-free transcription system and studied regulatory properties of the 5' upstream region of the IP3R1 gene by use of this system. Deletion analyses of the promoter revealed several cis elements that function significantly in brain nuclear extracts. Among those elements, sequences from -396 to -295 showed the most predominant cerebellum-specific positive function. Footprint analyses demonstrated a factor-binding region from -334 to -318, termed box-I, that contained an E- box consensus sequence. Electrophoretic mobility shift assay revealed CNS- related basic helix-loop-helix proteins for the box-I. Mutational studies using the function assay end competitive electrophoretic mobility shift assays demonstrated a good correlation between the box-I-binding factors and the activated transcription. Box-I-binding factors were present abundantly in adult mouse CNS, whereas their existence was restricted in embryonic and nonneural tissues. Transient chloramphenicol acetyltransferase assay for the IP3R1 promoter revealed the requirement of box-I in Neuro2a neuroblastoma cells. In the postnatal CNS, multiple basic helix-loop-helix factors are expressed abundantly, some of which are suggested to activate IP3R1 gene expression in the mammalian CNS.

本文言語English
ページ(範囲)476-484
ページ数9
ジャーナルJournal of Neurochemistry
69
2
DOI
出版ステータスPublished - 1997 8
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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