Denaturation of purple membranes at the air/water interface studied by SEM

Taiji Furuno, Hiroyuki Sasabe

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Fragments of purple membrane were spread at the air/water interface. The interfacial films were transferred onto a silicon wafer with time and initial coverage taken as parameters. The structure of the transferred films was examined with a scanning electron microscope. It was shown that both the size and the packing density of the membrane fragments decreased rapidly with time when the initial coverage was low, and that the intermembrane region was occupied by some substances. An assay by SDS polyacrylamide gel electrophoresis of a built-up film showed that bacteriorhodopsin molecules existed also in the intermembrane region. These results indicated that the denaturation of the purple membrane or the unfolding of bacteriorhodopsin occurred from the periphery of a membrane fragment at the air/water interface.

本文言語English
ページ(範囲)225-232
ページ数8
ジャーナルJournal of Colloid And Interface Science
147
1
DOI
出版ステータスPublished - 1991 11月
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 表面、皮膜および薄膜
  • コロイド化学および表面化学

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