Dendritic cells administered intrarectally penetrate the intestinal barrier to break intestinal tolerance via Th2-medeiated colitis in mice

Tango Handa, Takanori Kanai, Toshiro Sato, Yohei Mikami, Tomohisa Sujino, Atsushi Hayashi, Shinta Mizuno, Atsuhiro Matsumoto, Tadakazu Hisamatsu, Toshifumi Hibi

研究成果: Article査読

抄録

Intestinal lamina propria dendritic cells (LPDCs) in mice are known to extend dendrites between the intestinal epithelia and the luminal side when processing luminal antigens. We conducted intrarectal cell transfer experiments of bone marrow-derived dendritic cells (BMDCs) in mice to assess dendritic cell penetration of the intestine. Intrarectally administered GFP+ BMDCs localized in the colonic LP within 3h and the spleen within 12h after administration. 72h after administration, recipient C57BL/6 mice showed acute diarrhea, and administration of BMDCs (once weekly for 3 weeks) induced intestinal inflammation with increased numbers of recipient macrophages and CD4+ T cells exhibiting a Th2-mediated immune response. These results demonstrate that DCs actively communicate across the intestinal barrier, and highlight a potential technique for controlling colonic immune tolerance.

本文言語English
ページ(範囲)123-129
ページ数7
ジャーナルImmunology Letters
150
1-2
DOI
出版ステータスPublished - 2013 2 1

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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