Derivation of induced pluripotent stem cells from human peripheral circulating T cells

研究成果: Article査読

29 被引用数 (Scopus)

抄録

This unit describes a protocol for the generation of induced pluripotent stem (iPS) cells from human peripheral circulating T cells. Initially, human dermal fibroblasts and retroviral vectors were used to generate human iPS cells. Invasive approaches, such as skin biopsy, and genomic insertion of transgenes into the host genome are not appropriate for routine clinical application. Peripheral circulating T cells are readily available from blood samples of patients and healthy volunteers. For the efficient generation of human iPS cells, efficient introduction of the transgene into host cells is necessary. Using a combination of activated T cell culture and Sendai virus allows for the easy and efficient introduction of transgenes into activated T cells and the generation of human iPS cells without genomic integration of extrinsic genes. The T cell-derived iPS (TiPS) cells exhibit monoclonal T cell receptor (TCR) rearrangement in their genome, a hallmark of mature terminally differentiated T cells.

本文言語English
論文番号4A.3
ジャーナルCurrent Protocols in Stem Cell Biology
SUPPL.18
DOI
出版ステータスPublished - 2011 9

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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