Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange

Yuichiro Koide; Yasuteru Urano; Akira Yatsushige; Kenjiro Hanaoka; Takuya Terai; Tetsuo Nagano

doi:10.1021/ja900443b

Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange

Yuichiro Koide, Yasuteru Urano, Akira Yatsushige, Kenjiro Hanaoka, Takuya Terai, Tetsuo Nagano

研究成果: Article › 査読

67 被引用数 (Scopus)

抄録

A novel design strategy for controlling the fluorescence and photosensitizing ability of thiazole orange (TO) has been developed. The validity of this approach was demonstrated by the synthesis of a 5-galactosidase-activatable photosensitizer, PhoTO-Gal, in which fluorescence is simultaneously activated. PhoTO-Gal was demonstrated to kill HEK293 lacZ(+) cells, which express 5-galactosidase, but not HEK293 lacZ(-) cells, under light illumination. Such activatable photosensitizers should allow more refined PDT without the side effect of prolonged light sensitivity and should also be useful as tools for reporter enzyme expression-specific cell ablation.

本文言語	English
ページ（範囲）	6058-6059
ページ数	2
ジャーナル	Journal of the American Chemical Society
巻	131
号	17
DOI	https://doi.org/10.1021/ja900443b
出版ステータス	Published - 2009 5月 6
外部発表	はい

ASJC Scopus subject areas

触媒
化学 (全般)
生化学
コロイド化学および表面化学

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AU - Terai, Takuya

AU - Nagano, Tetsuo

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Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange

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